Relative apparent ATP synthesis rate of vesicles reconstituted with ATP synthase after base bath at RT. The rate is calculated based on the slope of the initial 100 s of ATP production over time, using the rate of C18 as the reference. s-ATP, single-chain fatty acids reconstituted with ATP synthase (gray); s-d-ATP, single-chain fatty acids and double-chain phospholipids reconstituted with ATP synthase (cyan); d-ATP, double-chain phospholipids reconstituted with ATP synthase (red); d-q-ATP, double-chain and quadruple-chain phospholipids reconstituted with ATP synthase (purple). Data are represented as mean ± SEM.
