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A schematic diagram of the experimental design. Tumor tissue from 1928zT2, M28zT2, or T4M828zT2 groups was obtained from NSCLC PDX models at the endpoint (day 21). Tumor tissues were prepared into single-cell suspension. Tumor-infiltrating CAR+(GFP+) CD8+T cells from the M28zT2 and T4M828zT2 groups and tumor-infiltrating CD8+T cells from the 1928zT2 group were sorted by FACS. These tumor-infiltrating T cells were then stimulated with CD3/CD28 monoclonal antibodies (mAbs) and subjected to functional experiments. Finally, the cytotoxicity, cytokine production, and T cell expansion of tumor-infiltrating CD8+T cells from the 1928zT2, M28zT2, and T4M828zT2 groups were evaluated. Graphics were created withBioRender.com(agreement numberQW27PPO9DF).

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