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Anti-TGF-β CAR vector (T28zT2), anti-GPC3 CAR vector (G28zT2), and anti-CD19 CAR vector (1928zT2) based on an anti-TGF-β scFv (US20140127230A1), anti-GPC3 scFv (GC33), or anti-CD19 scFv (FMC63), respectively. All contained expression cassettes encoding a human CD8 leader signal peptide, CD28, CD3ζ, and TLR2 signaling domain along with eGFP using 2A self-cleaving peptide (2A). The eGFP expression was used to monitor CAR-transduced cells.

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