Top, FI represents pCaMKII in spines. Bottom, the vertical axis represents the number of spines. The pseudocolor indicates pCaMKII FI/volume in each spine;n= 71 (shNEGA:ER(+)), 14 (shNEGA:ER(−)), 16 (shSEPT3:ER(+)), and 78 (shSEPT3:ER(−)) spines; 1-way ANOVA with Tukey’s multiple comparisons test.