Histochemical examination using phalloidin or antibodies for each septin subunit in control (left) and L-LTP (center) sides of the DG 45 min after beginning unilateral and strong high-frequency stimulation of the medial perforant pathway fibers in freely moving unanesthetized rats causing L-LTP. Right, the fluorescent intensity of phalloidin or each septin subunit quantified across the molecular and granule cell layers. Arrowheads indicate the difference in fluorescence intensities between control and L-LTP. GCL, granule cell layer; IML, inner molecular layer; MML, middle molecular layer; OML, outer molecular layer. Scale bar, 50 m.
