Confocal images of Tg(uas:LifeAct-GFP) animals that were injected with gfap:gal4-TA-tdTomnls demonstrating GLS astrocytes with a nucleus (magenta) that lines the edge of the spinal cord and has a relatively simple morphology compared to neuropil astrocytes. The arrow points to the surface astrocyte magnified in the inset (scale bar, 25 μm).