Similarity matrix (where a score of 1 describes maximum similarity) and multidimensional scaling plots show that Myoc+ astrocytes, while transcriptionally distinct, more closely resemble white matter astrocytes (seeFigure S1) when compared to gray matter astrocytes (reanalysis from Hasel et al.2). GM, gray matter; WM, white matter; GLS,glia limitans superficialis. Differential gene expression for both spatial transcriptomics and scRNA-seq was performed using a Wilcoxon rank-sum test, with adjusted pvalues (padj) < 0.05 being considered significant. The sagittal brain sections in (A) were downloaded from https://www.10xgenomics.com/resources/datasets.
