Expressing emc RNAi with esgTS-FO using transgenes KK108316 and JF02300 together increases the proportion of Dl+ cells (solid arrowheads) and EEs (empty arrowheads) and reduces that of ECs (asterisks) compared to controls. KK108316 and JF02300 together produced Dl+, NRE-lacZ+ cells, possibly dedifferentiating EBs (see Figures 6I and 6J) or cells with mixed ISC/EB identity or marker expression. emc RNAi also increases Dl expression.
