Bar Plot from Scientific Research

Overnight cultures of mNeon-Green-labeled H99 and unlabeled tps1Δ or tps2Δ were combined at a 1:1 ratio in RPMI 1640 medium with 165 mM MOPS (pH 7) and incubated at 37°C for 24 h in ambient air or with 5% CO2. Cell populations were characterized by flow cytometry, and the percentage of mNeonGreen-negative cells in 5% CO2 was normalized to that in ambient conditions to determine a competitive fitness score for each strain as indicated. Bars indicate mean of three biological replicates ± SD. The difference between CO2 and ambient conditions for each mutant was analyzed by unpaired Student’s t test (**p< 0.01,***p< 0.001).

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