Immunostaining analyses show reduced Smo translocation into choroid plexus cilia during embryonic and postnatal development (E14.5, P0, and P14). Pink arrows, Smo in ciliary axonemes; yellow arrows, Smo at ciliary base; white arrow, dispersed Smo in the cytosol; blue arrow, a CPEC without Smo staining.n= 3 for each developmental stage; E14.5 vs. P0,****p< 0.0001; E14.5 vs. P14,****p< 0.0001; P0 vs. P14, ns,p= 0.8938; one-way ANOVA and Sidak multiple comparisons tests.
