Workflow from Scientific Research

TWAS workflow to identify genes associated with predisposition to TGCTs. Significant genes were defined using a false discovery rate less than 0.01. Robust associations were identified using a gene permutation testp< 0.05. Genes were jointly modeled at each locus to identify conditionally independent genes (Bonferroni-adjusted joint-conditionalp< 0.05). Colocalization analysis was subsequently conducted using a posterior probability (PP4) threshold of >0.5 to identify genes likely influenced by a shared causal variant affecting both gene expression and TGCT risk.

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