Microscopy from Scientific Research

Left: maximum projection images of transgenic Fgfrb-EGFP polyps at indicated time points following physa amputation (top) and a schematic of the tomo-seq workflow (bottom). Green: Fgfrb-EGFP. Gray: DNA. Scale bar: 50 μm. Right: Fgfrb-EGFP fluorescence intensity distribution along the aboral-oral axis across the indicated time points. Shaded regions around each line represent the standard deviation. The plot below quantifies the physa-specific Fgfrb-EGFP fluorescence intensity across time points (unpaired Student’s two-tailed t test,p< 0.001:***;p< 0.01:**; n.s., not significant).

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