Maximum projection images of whole-mount HCRin situfor eight regional marker genes, including those shown in (E), with corresponding expression patterns in the tomo-seq data shown as histograms above each HCR image. Slices (x axis) in the histograms are ordered by pseudo-location, and a loess-fitted line represents the expected HCR pattern (refer toFigure S2). HCR intensity profiles are displayed using the pseudocolor palette fire. Scale bars: 100 m. See alsoFigure S1andTables S1andS2.
