VU increased residual depolarization at ZT3 (5 neurons, 4 animals;*p= 0.017, t test; t = 2.782; df = 12; d = 1.55). Bumetanide reduced residual depolarization at ZT15 (5 neurons, 4 animals;*p= 0.018, t test; t = 2.816; df = 10; d = 1.65). Error bars represent mean ± SEM.