Submitochondrial localization of oeTbMfnL-K141A::3Ty1 in PCF by immunofluorescence. Colocalization of oeTbMfnL-K141A::3Ty1 (anti-Ty1) with matrix mitochondrial threonine dehydrogenase (anti-Tdh) was analyzed by standard immunofluorescence after 5 days of induction (day 5 + Tet). n, nucleus; k, kinetoplast.