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Different PINK1 promoter constructs (PINK1-ARE-1-2-3, PINK1-ARE-2-3, PINK1-ARE-3) were co-transfected in HEK293T cells with the pRL-TK reporter gene (normalize transfection efficiencies) for 24 h. Data are expressed as a percentage of firefly luciferase activity (promoter constructs-transfected cells)/Renilla luciferase activity (pRL-TK-transfected cells) by Dual-Glo luciferase assay system and presented as the means SD of 3 independent experiments performed in triplicates.
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