Relative Gli1 mRNA level of kidney ( i ) and skin ( j ) from the Fab 6H3 treatment group compared to PBS treatment group. Fab 6H3 was generated with the Ab 6H3 purified from hybridoma cells. Each point was an average of 4 technical replicates of RT-PCR. Data are represented as mean ± SD ( n = 6). Two-sided t -test was performed between the PBS treatment group and Fab 6H3 treatment group by GraphPad Prism 10. P values are labeled.