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Schematic diagram of experimental determination of the virus entry steps affected by M1R-01. For the binding step, the VACV was mixed with Tri-M1R-01, added into the pre-cooled cells, and allowed for incubation at 4 °C for 1 h. Then, the cells were washed with cold PBS and harvested for qRT-PCR analysis of viral DNA copies. For the post-binding step, the VACV was first incubated with the target cells at 4 °C for 1 h, and then moved to 37 °C. Tri-M1R-01 or control isotype Nb was added at different timepoints, and plaques were counted at 48 h post-infection.
#Workflow#Flowchart#Schematic Diagram#Virus Entry#M1R-01#VACV#Tri-M1R-01#Cells#qRT-PCR#Viral DNA Copies#Plaque Assay
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