Gel from Scientific Research
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MEFs stably expressing TBC1D23-targeting shRNA (sh Tbc1d23 ), or control shRNA (shCT) were treated with DMXAA (25 μg/mL) for 30 min. Endosomes and heavy organelles (Golgi, endoplasmic reticulum, mitochondria, etc.) were isolated, and subjected to immunoblotting. EEA1 and golgin-97 were used to indicate endosomes and Golgi, respectively.
#Gel#MEFs#TBC1D23#shRNA#Control shRNA#DMXAA#Endosomes#Golgi#Endoplasmic Reticulum#Mitochondria#Immunoblotting#EEA1#Golgin-97
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