Quantifying the ratio of clustered protein fluorescence intensity to total fluorescence intensity within a cell ( n = 30). One representative experiment of at least three independent experiments is shown. Data analyzed by two-tailed t -test and shown as mean SD ( n 3). ns, not significant, p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.