Quantification of P-body number with or without puromycin treatment. Each dot represents the P-body number per field of view (FOV), with FOVs containing similar numbers of cells between conditions (no treatment, n = 16 FOVs; puromycin treatment, n = 19 FOVs). Error bars represent the mean s.d. **** P < 0.0001 (two-tailed t -test).