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Two AAV genomes are used: Genome A delivers a concatemerization-dependent barcode (ConcBC), and Genome B delivers the T7 RNA polymerase promoter. Concatemerization of these two genomes orients the T7 promoter and the ConcBC such that T7 RNA polymerase can transcribe the ConcBC. Genome B also contains a concatemerization-independent barcode (GenBC), driven by an SP6 RNA polymerase promoter. Both genomes carry a CAG-driven EGFP.
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