Illustration from Scientific Research

Schematic of AAV-Zombie. A barcode and phage RNA polymerase promoter are integrated into the AAV genome. While the cell is alive, the barcode is not transcribed. After fixation, in situ transcription of the barcode by phage RNA polymerase yields barcoded transcripts that can be detected by HCR-FISH. These transcripts serve as a proxy for the AAV genome.

DOI