HRA can be used to compare the distribution of parenchymal cells including endothelial, epithelial and muscle that compose the blood vessels, airways and gas exchanging functional lung structures and resident immune cells including macrophages, to local vasculature (VCCF visualization) in healthy (top) and diseased (bottom) lung using multiplexed immunofluorescence microscopy images with bronchiole (br) and an accompanying small pulmonary artery (pa). Scale bars, white 5 mm; red 200 m; and yellow 100 m. The graphs on the right show distance distributions for cell types present in the healthy lung (top) and diseased BPD lung (bottom); the violin plot (middle) shows a comparison between distance distributions for cell types common in both datasets. Datasets are on GitHub 81.
