Violin plots of the CTX and TH scores for each astrocyte subcluster identified from the CosMx ( f ; P values: top left, (2.3x 10}^{-13}) ; top right, (5.7x 10}^{-4}) ; bottom left, (2.2x 10}^{-3}) ; bottom right, (3.1x 10}^{-10}) ) data. Two-sided t -tests were conducted to determine the statistical significance of the CTX and TH scores of the astrocytes in each subcluster; *** P < 0.01.