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Strong activation of ethylene (ET) biosynthesis and ET-responsive genes at 24 HAP in the persistent synergid cell. Transcript levels are shown as FPKM values (means SD) of three biological replicates. For statistical analysis, count data at the gene level were analyzed with DESeq2 64 . Stage-to-stage comparisons were performed and corrected for multiple testing over all genes and cell stage comparisons using false discovery rate (FDR). Letters above bars indicate significant differences (adjusted P < 0.05). For ET biosynthesis genes, significant differential expressions (DEs) are shown (*log 2 FC > 1 and adjusted P < 0.05, ***log 2 FC > 1 and adjusted P < 0.001).
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