Synthesis of Ac-CoA relies on the rearrangement of the N- and the C-terminal domains of AcsA (NTD in cyan and CTD in red, respectively). Left and middle panels show that adenylate and thioester conformations of AcsA (PDB-IDs: 1T5D and 1PG3 , respectively 30 ). Right panel: The cryo-EM structure of the AcuA-AcsA complex (this study, PDB-ID: 9G7F ) shows that AcuA (blue) locks the CTD of AcsA in a position not suitable for catalysis. All structures were superimposed on the NTD of AcsA.
