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qPCR showed a specific knockdown in Ghsr mRNA in the EW ( p < 0.0001), but not VTA ( p = 0.4256; n’s=11 Sc-ShRNA [7 F, 5 M pooled], 13 Ghsr -ShRNA [6 F, 7 M pooled]; males=blue, females=green). Analysed by two-tailed unpaired t -test.
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