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Splenic T cells from Sham mice ( n = 4) were co-cultured with plasma cells (PCs) purified from CLP mice ( n = 8) with or without HTS Transwell inserts (T cells in the bottom of the well and PCs on the top of the membrane) at a 1:1 ratio for 72 h, and stimulated by anti-CD3/CD28 antibodies-coated beads. T-cell proliferation was measured among viable cells by flow cytometry. A non-parametric Wilcoxon paired test was used to compare proliferation in the presence of PCs with or without the inserts.

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