Violin Plot from Scientific Research

Levels of BubR1-pT620, Bub1-pT609, Knl1-pMELT and Knl1-pRVSF at unattached kinetochores, in nocodazole-arrested HeLa-FRT cells expressing directSLiMs LIE1/LIE9 and treated with vehicle for 20 min. Note that the distributions of directSLiM LIE1 and directSliM LIE9 are also shown in Supplementary Fig. 4D . Kinetochore intensities from 20 to 30 cells, 2–3 experiments. Source data are provided as a Source Data file. Data information: Kinetochore intensities in B, D and E are normalized to directSLiM LIE1 vehicle condition. Violin plots show the distributions of kinetochore intensities between cells. For each violin plot, each dot represents an individual cell, the horizontal line represents the median and the vertical one the 95% CI of the median, which can be used for statistical comparison of different conditions (see Methods).

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