Violin Plot from Scientific Research

Effects of PP2A-B56 inhibition on the levels of BUBR1-pT620 at unattached kinetochores (top) and on the mitotic cell fate after nuclear envelope breakdown (NEBD, bottom) in HeLa-FRT cells with/without directSLiMs LIE1-7/AAA expression and treated with vehicle or rapamycin. Top graph: Kinetochore intensities from 20 nocodazole-arrested cells, 2 experiments except HeLa-FRT, DMSO: 10 cells. The treatment with vehicle/rapamycin was performed for 20 min prior fixation. Bottom graph: heatmap showing the mean frequencies of cell fate after NEBD in each condition: 2 experiments, 17–50 cells per condition per experiment (see also Supplementary Fig. 4A ).

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