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Experimental design: mitotically arrested directSLiM LIE1 and HeLa-FRT cells were treated with rapamycin, rapalog or vehicle before harvest ( n = 3 for each condition). Following cell lysis, proteins were digested, and peptides were TMT-labeled. Next, phosphopeptides were enriched with Ti-IMAC magnetic beads, followed by high-pH reversed-phase fractionation and measurement by LC-MS.

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