Microscopy from Scientific Research

Representative example immunofluorescence images to show the effects of PP2A-B56 inhibition on the levels of FLAG-PPP2R1A, BUBR1-pT620 and BUBR1 at unattached kinetochores in nocodazole-arrested HeLa-FRT cells expressing the directSLiMs LIE1/AAA and treated with vehicle or rapamycin for 20 min. Kinetochore intensities from 20-40 cells, 2–4 experiments. The insets show magnifications of the outlined regions. Scale bars: 5 μm. Inset size: 1.5μm

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