Normalized numbers of smFISH spots detected in MB γ axons of wild-type and imp- Δ PLD mutants. Two to four biological replicates were performed, and the mean value of each is indicated as a triangle. Data points were color-coded based on the replicate they belonged to. 15098 stands for cg15098 . **, P < 0.01; ***, P < 0.001 (Two-tailed Mann–Whitney tests). n.s. stands for not significant. Two outlier data points (one for arc1 and one for pabp ) were omitted from the graph (but considered to calculate the mean and to perform the statistical tests). Exact P values: <0.0001 ( arc1 ), 0.0058 ( eIF4A ), 0.0008 ( cg15098 ), <0.0001 ( prof ), 0.0035 ( act5C ), 0.0719 ( lk6 ), 0.0902 ( crc ), 0.9330 ( bnb ), 0.4527 ( eIF4-E1 ), 0.1925 ( pabp ). Numbers of brains analyzed for control and imp-ΔPLD conditions respectively: 24 and 25 ( arc1 ), 21 and 19 ( eIF4A ), 21 and 20 ( cg15098 ), 19 and 20 ( prof ), 20 and 25 ( act5C ), 24 and 22 ( lk6 ), 18 and 19 ( crc ), 22 and 19 ( bnb ), 22 and 22 ( eIF4-E1 ), 22 and 17 ( pabp ). Source data are provided as a Source Data file.
