Quantification of the number of smFISH spots in MB axons for the candidate Imp-bound mRNAs analyzed. Three to four biological replicates were performed, and data from each replicate was labeled with different colors. Bars represent mean values. pabp mRNA distribution was analyzed using a GFP protein-trap knock-in line. Four outlier data points (two for arc1 and two for eiF4A ) were omitted from the graph but considered to calculate the mean. Numbers of brains analyzed: 24 ( arc1 ), 21 ( eIF4A ), 21 ( cg15098 ), 22 ( pabp-gfp ), 19 ( prof ), 22 ( eIF4E-1 ), 18 ( act5C ), 22 ( bnb ), 18 ( CRC ).
