Lymph node CD4 + T cells of the indicated genotypes were activated on plate-bound anti-CD3 and anti-CD28 antibodies at the indicated doses. Graphs show the percentage of Ki67+ cells at 24 h ( j ) and the percentage of cells that had divided at 48 h ( k ) ( n = 9). Dots represent biological replicates. Data are pooled from 2 independent experiments ( a, c, e, h-k ), from 1 experiment representative of 2 ( g ) or from a single experiment (d ). WNKi, WNK inhibitor (WNK463); AQP3i, AQP3 inhibitor (DFP00173). Statistical analysis with the 2-sided Mann-Whitney test ( a, e, g-i ), Kruskal-Wallis test with correction for multiple comparisons ( c ) or 2-way ANOVA ( j ). * 0.01 < p < 0.05; ** 0.001 < p < 0.01. *** 0.0001 < p < 0.001. **** p < 0.0001. Source data are provided as a Source Data file.
