Orthogonal sections of 3D images of mouse nave CD4 + T cells constitutively expressing tdTomato-CAAX, stimulated for 1 h on plate-bound anti-CD3 and anti-CD28 antibodies with WNKi or vehicle only. Grey scale represents the tdTomato fluorescence intensity. White dashed lines show positions of the orthogonal slices which were taken at the widest and highest point of the cell. Blue dashed line represents the outline of the vehicle control cell superimposed on the WNKi-treated cell. Green arrows show the location of the cell/slide interface in the XZ and YZ sections. Scale bars, 5 m.
