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FBNs expressing miniSOG were held at –80 mV in the intervals of 0–10 and 30–40 min (except during the voltage protocols required to measure I A ) and repeatedly step-depolarized to +10 mV (3 ms, 10 Hz) between 10 and 30 min. Nine-minute exposures to blue light (between the 0- and 10-min and the 30- and 40-min time points) increase the fast and slow inactivation time constants of I A above their pre-illumination baselines (b ; blue versus grey shading); a series of depolarization steps between 10 and 30 min reverses this increase ( b ; yellow shading; τ fast : P < 0.0001; τ slow : P = 0.0008; mixed-effects model; examples of peak-normalized I A evoked in the same dFBN by voltage steps to +30 mV in a ).
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