dFBNs were held at –80 mV, except during the voltage protocols required to measure I A . At 10 min after break-in, the inclusion of 50 M 4-ONE, but not of 200 M 4-HNE, in the intracellular solution increases the fast and slow inactivation time constants of I A from control to sleep-deprived levels, provided dFBNs express catalytically competent Hyperkinetic ( fast : P < 0.0001; slow : P < 0.0001; Kruskal–Wallis ANOVA). Columns show population averages; dots represent individual cells; n , number of cells; asterisks indicate significant differences ( P < 0.05) relative to the 0-min time point or control levels in planned pairwise comparisons by Holm–Šídák or Dunn’s test. For statistical details see Supplementary Table 1 .
