Example fluorescence (top) and positive-ion SMALDI-MS images (bottom) of cryosections containing dFBNs marked with mCD8::GFP. The sections were cut from rested (left) or sleep-deprived brains (right). SMALDI-MS images show, from top to bottom, the spatial distributions of phosphatidylinositol 18:2/20:2 ( m / z 887.5612, [M+Na] + ), phosphatidylserine 18:3/20:5 ( m / z 826.4618, [M+Na] + ), phosphatidylcholine 18:0/18:1 ( m / z 788.6140, [M + H] + ), phosphatidylcholine 18:3/18:3 ( m / z 778.5345, [M + H] + ), phosphatidylethanolamine 18:1/18:1 ( m / z 744.5536, [M + H] + ) and phosphatidic acid 18:2/20:3 ( m / z 723.4932, [M + H] + ). Scale bar, 200 m.
