Workflow from Scientific Research

Schematic of custom-built confocal micro-Raman system. L1 and L2, lenses; LPD, long-pass dichroic beam splitter; SP, short-pass filter; LP, long-pass filter; PH, pinhole; TL, telescope lens; EMCCD, electron-multiplying charge-coupled device. The setup is featured by underfilling the back aperture of a 20 objective lens (effective numerical aperture of about 0.4) and still capturing the scattered Raman signal with a large collection efficiency. This configuration yields a confocal volume of about 2 m x 2 m x 10 m. An optical pinhole size of 40 m is chosen based on the first minimum of the Airy disk from a point source.

DOI