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CD4 + T cells of the indicated genotypes or in the presence of the indicated inhibitors were cultured for 1 h in the presence or absence of plate-bound anti-CD3epsilon and anti-CD28 antibodies. Graphs show the percentage of cells that were p-ERK high and p-ERK mean fluorescence intensity normalized to stimulated controls cells (set to 1) as determined by flow cytometry, gating as in Supplementary Fig. 5d, e ( n = 8).

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