Cells were pulsed with EdU for 10 min and incorporation of EdU (d, i ) was used to calculate the rate of EdU incorporation (ΔEdU) by subtracting the average EdU MFI in G1 and G2/M phase cells from the average EdU MFI in S phase ( e, j ) using the gates shown. Sample numbers: a , n = 10; c , Wnk1 +/- RCE: n = 9, Wnk1 -/- RCE: n = 8; e, n = 5; f , n = 7 except Oxsr1 +/+ Stk39 +/+ RCE at day 0, n = 6; h , Oxsr1 +/+ Stk39 +/+ RCE: n = 7, Oxsr1 +/+ Stk39 T243A/T243A RCE: n = 5; j , Oxsr1 +/+ Stk39 +/+ RCE: n = 7, Oxsr1 +/+ Stk39 T243A/T243A RCE: n = 6. Each point represents a different mouse; horizontal lines or columns show median; data pooled from 2 independent experiments ( a, c, f, h, j ) or from 1 experiment representative of 2 ( e ) experiments. Statistical analysis with the 2-sided Mann-Whitney U test. * 0.01 < p < 0.05; ** 0.001 < p < 0.01, **** p < 0.0001. Source data are provided as a Source Data file.
