Naive CD4 + CTV-labelled T cells of the indicated genotypes ( c–f, i, j ) or treated with WNKi ( g, h ) were activated on plate-bound anti-CD3 and anti-CD28 antibodies for indicated times. CTV dilution ( c, e, g, i) was used to determine the percentage of cells that had divided ( d, h, j, n = 5; f , Wnk1 +/- RCE: d0, 2, 3, n = 6; d1, n = 5. Wnk1 D368A/- RCE: d0, 3, n = 7; d1, 2, n = 6). WNKi, WNK inhibitor (WNK463). Each point represents a different mouse; horizontal lines show median; data are from 1 experiment representative of 2 ( b, h ) or 3 ( d, j ) experiments, or pooled from 2 independent experiments ( f ). Statistical analysis with the 2-sided Mann-Whitney U test. *0.01 < p < 0.05; **0.001 < p < 0.01. Source data are provided as a Source Data file.
