Scatter Plot from Scientific Research

Open access visualization of Scatter Plot, Protein Synthesis Assay, HPG Incorporation, Alexa Fluor 488, Wild-Type Fibroblasts
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Nascent protein synthesis assay using HPG incorporation followed by labeling using a click reaction with Alexa fluor 488 (AF 488) in WT fibroblasts expressing a BAF WT or BAF A12T construct ( D ). AF 488-HPG intensity was quantified using the high-content microscope in 3 independent experiments, each measuring 1000 cells in 3 wells per cell line, graphed as a Superplot with data from different experiments indicated in different colors and larger symbols indicating each experiments average. AF 488-HPG intensity was normalized to the WT cell line. Mean values were analyzed using paired two-tailed t-test ( D ).

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