MELs produced from indicated strains were analyzed by TLC. Different MEL variants, which differ in their acetyl-moieties are specified MEL-A—MEL-C. Ustilagic acids (UAs) are a different class of glycolipids produced by U. maydis but not synthesized in peroxisomes 39 . Mac2 is another acyltransferase involved in MEL production, which has overlapping functions with Mac3 and was deleted to facilitate analysis.