Quantification of colocalization data derived from epifluorescence microscopy depicted in Fig. S3b based on three independent biological replicates. Samples were analyzed directly after (t0), 3 h after (t3), and 6 h after glucose addition (t6). Quantifications show Pearson’s correlation coefficients of GFP and mCherry signals for indicated strains. Center line, median. Significance assessed by an unpaired, two-sided Student’s t -test. **** refer to a p value lower than or equal to 0.0001.