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S.cerevisiaetransformants were cultured in the presence of K2 toxin, and the OD600was recorded after 24 h. WT K2, wild-type K2 sequence; EP, empty plasmid control. The numbers on the x axis indicate the first and final deleted codons of each deletion construct. Note that the first construct also omits the start codon at position 1. A separate construct was cloned that retains the start codon (seeFigures S1C–S1E).
#Bar Plot#Error Bars#S. cerevisiae Transformants#K2 Toxin#Optical Density#Wild Type K2 Sequence#Empty Plasmid Control#Deletion Construct
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