The percentage of fully fed (left) and partially fed (center) Ae. aegypti female mosquitoes fed on mouse serum or ultrafiltrated mouse serum of molecular weight less than 10 kDa containing a final concentration of 0.1 mM ATP. Sample sizes were as follows: ATP only (n= 3), ATP with serum (n= 5), and ATP with ultrafiltrated serum (n= 6). Body weight of each mosquito is shown (right). Sample sizes were as follows: ATP only (n= 72), ATP with plasma (n= 94), and ATP with serum (n= 92). The weight of a control unfed mosquito that has been given nothing by membrane feeding (n= 40) is also shown. All data are presented as truncated violin plots, with median indicated by horizontal lines and the bounds of the violin corresponding to the range. The p values were determined using 1-way ANOVA with Tukey’s multiple comparison test to test among groups for (C) and (D), or unpaired t test with Welch’s correction for (A).
