Gel from Scientific Research

Abrogation of CDK1 expression induced by inhibitors of RAF, MEK, and CDK4/6, verifying drug specificity targeting the RAS/RAF/MEK/ERK pathway in MDMs under normoxic conditions. Inhibitors used were U0126 (10 μM), palbociclib (pal; 1 μM), Tak632 (Tak; 2 μM), and AZ628 (AZ; 1 μM). Shown is a representative western blot of cell lysates harvested post 2 days of incubation under hypoxia or with addition of the inhibitors under normoxia.

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